Experimental
Techniques
Lamarck is dedicated to the core experimental technologies in life science research, systematically covering
key fields such as protein detection, cell analysis, and molecular interaction. It provides professional
interpretations of the core principles, operational points, and application scenarios of various
technologies
from a professional perspective, offering comprehensive professional support for researchers from technology
selection evaluation to experimental protocol optimization, and facilitating accurate and efficient
scientific
research practice.
Western Blot（WB）
Separates proteins via gel electrophoresis and combines antigen-antibody specific reaction to achieve qualitative and semi-quantitative analysis of target proteins, serving as a classic technique for protein expression detection.
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Immunohistochemis-try (IHC)
Uses specific antibodies to label target antigens in tissue sections, and localizes the distribution of target proteins in tissues through chromogenic reaction, suitable for pathological diagnosis and tissue-level protein expression research.
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Multiplex Immunohisto- chemistry（mIHC）
Based on TSA (Tyramide Signal Amplification) technology, it enables simultaneous detection of multiple targets on a single tissue section. Combined with fluorescent labeling, it achieves multi-index visual analysis, facilitating research on complex mechanisms such as the tumor microenvironment.
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Immunofluorescence (IF)
Labels antibodies with fluorescein, observes fluorescent signals via fluorescence microscope to achieve accurate localization and expression analysis of target antigens in cells or tissues, with the advantages of high specificity and visualization.
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Co-Immunoprecipitation（Co-IP）
Captures target proteins with specific antibodies and precipitates their in vivo-bound protein complexes simultaneously, enabling efficient identification or verification of physiological interactions between proteins, and serving as a core experimental technique for analyzing the composition and function of protein complexes.
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Enzyme-Linked Immunosorbent Assay （ELISA）
Converts antigen-antibody binding signals into quantitative data through enzyme-catalyzed substrate chromogenesis, with high sensitivity and specificity, enabling quantitative detection of biomolecules such as antibodies, antigens, and cytokines.
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Flow Cytometry Assay（FCM）
Combines fluorescence labeling with flow cytometer to perform rapid sorting, counting and multi-parameter (e.g., cell phenotype, apoptosis status) analysis of cells or particles, and is a core technique in cell biology and immunology research.
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Chromatin Immunoprecipitation Assay（ChIP）
Captures DNA-bound proteins (e.g., transcription factors, histones) with specific antibodies, and analyzes the bound DNA sequences through subsequent detection, which is used for the research on DNA-protein interactions and gene expression regulation.
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