Fss^TM Rabbit monoclonal antibodies （mAbs）: Multidimensional Four-tier Specific Precision Screening System – Rabbit Genetically Engineered Antibodies

Lamarck Fss^TM Rabbit mAbs, supported by a Multidimensional Four-tier Specific Precision Screening System, implement a progressive quality control process from the immunization source to antibody purification. This system enables precise regulation of high specificity and high affinity of antibodies, fostering the development of high-quality Rabbit Genetically Engineered Antibodies.

Fss^TM Rabbit mAbs Platform

Tier 1: Dual Detection of Immunization and Serum – Laying the Foundation for Specificity

Specific peptides or eukaryotic proteins are used as immunogens to accurately activate the rabbit immune system for targeted antibody production. Simultaneously, post-immunization rabbit blood samples are collected, and dual verification (endogenous sample-specific detection and serum titer detection) is performed to confirm the antibody specificity and reactive activity at the serum level. This step screens out immunized individuals with high potential for producing high-quality antibodies.

Tier 2: Double-Positive B Cell Sorting – Targeting Core Secretory Cells

Entering the B cell screening phase, double-positive flow cytometry sorting technology is employed to accurately identify and isolate B cells that are both IgG+ (expressing surface IgG) and antigen-specific+. This directly targets the core cell population capable of secreting the target antibody, significantly improving the efficiency of subsequent screening.

Tier 3: Multi-Dimensional Verification of Single B Cells – Enhancing Antibody Specificity

The sorted single B cells are cultured individually. Multiple classic experimental techniques – including ELISA (Enzyme-Linked Immunosorbent Assay), WB (Western Blotting), IF (Immunofluorescence), and IHC (Immunohistochemistry) – are used for multi-dimensional verification and identification of the specificity of antibodies secreted by single B cells. Cells with non-specific reactions are eliminated to ensure antibody targeting.

Tier 4: Recombinant Expression and Multi-Dimensional Comprehensive Detection – Ensuring Antibody Quality

The antibody variable region genes from the screened positive B cells are cloned and recombined, then introduced into mammalian cells for efficient expression. A multi-dimensional comprehensive specificity verification protocol (including ELISA, WB, IHC, IF, FCM (Flow Cytometry), IP (Immunoprecipitation), and ChIP (Chromatin Immunoprecipitation)) is applied to fully test the recombinant antibodies. Finally, through optimized cell culture, high-efficiency antibody expression, and refined purification, Rabbit Genetically Engineered Antibodies with high purity, high specificity, and high affinity are obtained.

Antibody Core Product Advantages

Superior Specificity: Backed by the "Multidimensional Four-tier Specific Precision Screening System" and "Five-Pillar Strategies", quality control is implemented throughout the process from immunization to antibody verification, ensuring high antibody specificity and affinity.

Excellent Quality: Fully validated with endogenous samples, reaching IP-grade standards. It features ultra-high dilution ratio and high sensitivity, compatible with multi-scenario applications (WB, IHC, IF, IP, ELISA, FCM, ChIP) with stable and reliable results.

High Purity Guaranteed by Advanced Process: Antibody variable region genes from positive B cells are cloned, and recombinant production is carried out using a serum-free eukaryotic expression system. Combined with affinity and ion-exchange chromatography purification, high-purity antibodies are obtained.

Strong Batch Stability: Standardized protocols for gene cloning, expression, and purification effectively control production variations, ensuring consistent antibody performance across batches and guaranteeing experimental reproducibility.

High Recognition in Scientific Research: Widely applied in the field of scientific research, with related findings published in multiple academic papers, providing strong tool support for research work.

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