Pathological-Grade Primary Antibodies for IHC

Developed to meet the core goal of IHC experiments on human tissue samples, these antibodies cover the entire R&D process from immunogen design and positive cell line screening to antibody function validation. Guided by top international monoclonal antibody preparation experts throughout, they adhere to clinical testing standards and their performance is compared with that of international pathological antibody brands.

I. Precision R&D: Focused on IHC

1. Immunogen Design: Core Guarantee for Specificity

The selection and design of immunogens are critical to the success of antibody R&D. Synthetic peptide fragments are preferred as immunogens, under the guidance of top international monoclonal antibody experts. This ensures clear antigen epitopes, enhances antibody specificity and targeting from the source, and reduces non-specific binding interference in IHC experiments.

2. Cell Line Screening: Targeting High-Efficiency Secretory Cells

Guided by IHC experiment requirements, positive mice and B cells that secrete the target antibody are accurately screened via IHC detection on human tissue samples. On this basis, hybridoma cell lines are cultured and screened step-by-step to obtain high-quality cell lines that stably and efficiently secrete the target antibody, laying a foundation for subsequent antibody preparation.

3. Antibody Preparation and Preliminary Testing: Balancing Purity and Activity

The screened high-quality hybridoma cell lines are cultured in mouse peritoneal cavities, and high-purity monoclonal antibodies are obtained through ascites purification technology. Simultaneously, antibody purity testing, isotype identification, and IHC detection are completed.

II. Clinical-Grade Quality Control: Aligned with Top International Standards

Comprehensive Quality Control, Aligned with Clinical Standards

A verification system is established based on the strict requirements of clinical pathological testing. It not only conducts multi-dimensional checks on core indicators of purified antibodies (such as purity, isotype, and IHC performance) to ensure stable and uniform antibody performance, but also verifies via parallel control experiments with well-known international pathological antibody brands. Through horizontal comparison of IHC results on human tissue samples, the detection effect is confirmed to be consistent with and even superior to top international standards.

Target: CEA

Tissue: human rectal cancer  

Target: CK-pan 

Tissue: human oral squamous cell carcinoma  

Target: CD31 

Tissue: human liver

Outstanding High Specificity, Ensuring Accurate Diagnosis

The antibodies exhibit extremely high specificity in recognizing target antigens, enabling precise binding to specific proteins and antigen epitopes in tissues/cells. They rarely cause non-target cross-reactions, effectively avoiding "false positives" and "false negatives", and are key to distinguishing normal/lesioned tissues and clarifying pathological classification.

Adaptable to Complex Samples, Compatible with Mainstream Technologies

Addressing challenges in pathological experiments (such as diverse sample types including FFPE, frozen tissues, and cell smears, difficult antigen retrieval, and sample degradation), the antibodies are specially designed and verified to withstand pretreatment processes like high-temperature antigen retrieval and dewaxing/deparaffinization. They can effectively bind to targets even in samples with low antigen expression, and are compatible with mainstream pathological detection technologies such as IHC and IF.

Strong Batch Stability, Reproducible Results

Strict batch quality control (including titer detection, specificity verification, and stability testing) is implemented during production. Differences in titer and binding capacity between different antibody batches are minimal, ensuring consistent detection results across different times and operators. This meets the "standardized and reproducible" requirements of pathological experiments and avoids diagnostic deviations caused by batch fluctuations.

Verified in Clinical Scenarios, Improving Experimental Efficiency

Verified with clinical samples (e.g., comparison with tissue sections of known pathological results), the antibodies provide clear pathological application data (such as positive expression patterns in diseased tissues and recommended dilution ratios), improving experimental efficiency.